|Intestinal microflora modified by Candida kefyr reduces the susceptibility to experimental autoimmune encephalomyelitis|| |
|K Takata1, T Tomita2, T Koda1, T Okuno1, JA Honorat1, M Kinoshita3, M Takei2, K Hagihara2, H Mochizuki1, S Sakoda4, Y Nakatsuji1|
|1Osaka University, Neurology, Suita, Japan, 2Kyorin Pharmaceutical Co., Ltd., Shimotsuga, Japan, 3Osaka University, Laboratory of Immune Regulation, Suita, Japan, 4National Hospital Organization Toneyama, Neurology, Toyonaka, Japan|
|Background: Diet is recently drawn attention as a potential risk factor of developing autoimmune diseases. Especially, the rapid increase in MS incidence in Japan seems to be attributed to a change of dietary habit. Although several dietary risks and benefits of probiotics have been suggested in animal models, little is known about the effects of dietary yeast on health.|
Objectives: The aim of our study is to examine the effects of dietary yeast on experimental autoimmune encephalomyelitis (EAE).
Methods: We chose 11 kinds of yeast which are contained in diet, then investigated the effects on EAE by oral administration. CD4-positive T cells in intestinal lamina propria and mesenteric lymph nodes (MLN) were analysed by FACS, and cytokine production from explants of intestine was assessed. The effects of Candida kefyr (C. kefyr) to intestinal microflora were analyzed by T-RFLP analysis of feces. Microflora transfer was performed by oral administration of diluted cecal contents after treatment with antibiotics cocktail.
Results: In yeast we examined, only C. kefyr exhibited significant clinical amelioration of EAE and the antigen-specific production of IFN-gamma and IL-17 by inguinal lymph nodes was decreased. Histological examination revealed the reduced inflammatory cellular infiltration into the central nerve system. The number of CD4-positive IL-17-producing cells was reduced in intestinal lamina propria. Intestinal tissue explant culture revealed that production of IL-6 was significantly suppressed in C. kefyr-treated group. In MLN, the number of Treg cells and CD103-posivtiv regulatory dendritic cells was significantly increased in C. kefyr-treated group. The analysis of 16s-rRNA revealed the increased Lactobacillales and decreased Bacteroides/Prevotella ratio compared to control flora. Transfer of intestinal microflora of C. kefyr-treated mice reproduced decreased Bacteroides/Prevotella ratio, and ameliorated EAE.
Conclusions: The effects of oral administration of yeast were varied depending on speices, and C. kefyr suppressed EAE by modifying microflora. Our findings suggest that dietary intake of C. kefyr may influence systemic immune state and has a beneficial effect on MS.
M.D. Ph.D Kazushiro Takata , Osaka University , Suita , JP
Assigned in sessions:
12.09.2014, 14:45-16:15, Poster viewing, poster sessions, P2, Poster Session 2 (P491-P981) and Coffee Break, Hall C