FREE PAPER PRESENTATIONS Methods: Explants of porcine retinae were cultivated for 4 and 8 days. Degeneration through 300µM CoCl2 (for 48h) and treatment with the iNOS- inhibitor 1400W (for 72h) were applied simultaneously from day one. Three groups were compared: control, CoCl2, and CoCl2+iNOS-inhibitor. At days 4 and 8, retinal ganglion cells (RGCs), bipolar, and amacrine cells were ana- lyzed via immunohistology and qRT-PCR. Furthermore, the influence on the glia cells and markers (HIF1α, HSP70, iNOS) involved in hypoxic/stress processes were evaluated. Results: Treatment with CoCl2 resulted in a significant loss of RGCs al- ready after 4 days (p=0.0002), which was counteracted by the iNOS-inhibitor (p=0.021). After 8 days the CoCl2 group displayed a significant loss in ama- crine cells (p=0.0003) and downregulation of PVALB mRNA expression (2.8- fold; p< 0.05). A drastic reduction in bipolar cells was observed after 8 days (p=0.0001), which was prevented by 1400W (p=0.001). A significant decrease of microglia (p=0.0001) was found in the CoCl2 group after 4 and 8 days, which could not be prevented by the iNOS-inhibitor. CoCl2 induced a significant increase in the mRNA expression of HSP70 (p=0.008), HIF1α, and iNOS (p=0.045). Again, this effect was counteracted by the iNOS- inhibitor. Conclusions: CoCl2 induces strong degeneration in porcine retinae by mim- icking hypoxia, damaging RGCs, amacrine, bipolar and microglia cells. Treatment with the iNOS-inhibitor counteracted these effects to some extent and clearly prevented the loss of retinal ganglion and bipolar cells. Hence, the iNOS-inhibitor 1400W seems to be a very promising treatment for glaucoma. FP09-04-GLA Decreased macular vessel density measured by optical coherence tomography angiography is associated with a reduced photopic negative response in glaucoma patients Cutolo C.A.1,2, Saccà S.3, Bonzano C.1,2, Ferrari D.3, Traverso C.E.1,2 1IRCCS Policlinico San Martino, DiNOGMI, Genova, Italy, 2University of Genova, Clinica Oculistica, Genova, Italy, 3San Martino Hospital, Genova, Italy Purpose: To investigate the association between the photopic negative re- sponse (PhNR) and structural/angiographic optical coherence tomography (OCT) parameters in glaucoma patients. Methods: This cross-sectional study enrolled patients with primary open angle glaucoma (POAG). All patients completed a full ophthalmological ex- amination. One eye per patients underwent optical biometry, OCT, OCT an- giography (OCT-A) and, full-field electroretinography to analyze the PhNR. OCT measurements: ganglion cell layer plus inner plexiform layer thickness (GCL+) and circumpapillary retinal nerve fiber layer (cpRNFL). Macular OCT-A measurements: vascular density of the superficial retinal layer (vd- SRL) and vascular density of the deep retinal layer (vd-DRL). Standard auto- mated perimetry (SAP) was also performed. Results: The study population consisted of 28 POAG patients. The PhNR sig- nificantly correlates with GCL++ thickness and vd-SRL (p=0.02 and p=0.01, respectively) but not with cpRNFL, SAP or, vd-DRL. Multivariate linear re- gression analysis, while controlling for the potentially confounding effect of age, central corneal thickness (CCT), axial length showed that each 10 µm decrease in GCL+ thickness was associated with a 0.90 µV decrease in the PhNR (p=0.006) and that each 1% decrease in vd-SRL was associated with a 0.35 µV decrease in the PhNR (p< 0.001). The multivariate regression also adjusted for the effect of SAP showed that vd-SRL was independently associated with the PhNR (R2=0.677, p<0.001). When controlling for the same variables, GCL+ wasn’t independently associ- ated with the PhNR (p=0.143). Conclusions: Our study confirms the significant relationship between OCT structural measurements and PhNR. Moreover, we showed a significant as- sociation between macular vascular density and the PhNR. FP09-05-GLA The increased epiretinal membrane frequency in patients with glaucomatous optic atrophy Ozkan Aksoy N., Cakır B., Özmen S., Celik E. Sakarya University, Sakarya, Turkey Purpose: To investigate the epiretinal membrane (ERM) frequency in patients with glaucomatous optic atrophy (GOA). Methods: The medical records of patients with glaucoma were investigated and the records of patients with GOA were recruited.The macular ERM was assessed by using high definition images obtained from enhanced depth opti- cal coherence tomography (OCT) and classified morphologically based on inner - outer macular thick- ness, inward projection of outer layers, foveal changes. The mean age, gender, the mean, fragmented retinal nerve fiber layer (RNFL) thicknesses, rim, disc area, mean, vertical cup-to-disc (c/d) ratio, the mean cup volume, the mean, fragmented ganglion cell-inner plexiform layer (GC-IPL) thicknesses mea- sured by OCT and mean deviation, pattern standard deviation were noted. Results: 88 of 68 patients were recruited. The mean age of 29 female, 39 male patients with GOA were 70,±9,4 years. The history of ocular surgery was present in 55 eyes. 45 eyes (51,1%) was diagnosed as ERM. 15 eyes (17%) were type 1A, 17 eyes (19,3%) were type 1B, 8 eyes (9,1%) were type 1C and 5 eyes (5,7%) were type 2B. The mean, superior, inferior, nasal,temporal RNFL thicknesses were 56±7 µ, 62,29±10,38 µ, 60±10,47 µ, 55,11±9,27 µ, 46,41±11,89 µ, respectivey. The mean, superior, inferior GC-IPL thicknesses were 56,4±9 µ, 57.14±12,09 µ, 55,79±11,56 µ, respectively. The mean MD and PSD were -22,56±5,9 and 10,5±2,36, respectively. The mean cup volume, rim, disc area, mean, vertical c/d ratio were 0,59±0,27 mm3, 0,6±0,2 mm2, 2,03±0,38 mm2, 0,81±0,07 and 0,81±0,08, respectively. Conclusions: The frequency of ERM in patients with GOA was as 51,1%. This result might be altered by the presence of previous surgeries but less than %10 frequency were reported in patients who underwent cataract surgery. So this frequency is a unique result which should be discussed and was needed further detailed studies. FP09-06-GLA The correlation between intraocular pressure and systemic factors in Chinese: population-based study Liu X.1, Jin C.2, Yi N.2 1Tsinghua University, School of Clinical Medicine, Beijing Tsinghua Changgung Hospital, Beijing, China, 2Meinian Institute of Health, Beijing, China Purpose: To investigate the relationships between intraocular pressure (IOP) and Systemic factors, such as age, gender, body mass index (BMI), blood pressure (BP), and metabolic syndrome risk factors in Chinese. Methods: Data from OneHealth Healthcare Screening Program around China in 2017 were utilized. We recorded the values of all healthy adult aged 18 and over for sex, age, BMI, systolic blood pressure (SBP), diastolic blood pressure (DBP), heart rate (HR), fasting blood glucose (FBG), total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low-density Congress of the European Society of Ophthalmology (SOE) 13–16 June, 2019, Nice, France - Abstract Book 29