|PT03 Transplantation: Science (Immunology) (ePoster)
|Selection of ePoster Presentations from Abstract Submissions
|PT03-05 ||Neutrophil CD 64 - Is it an Accurate Marker for Predicting Sepsis in Post LDLT Scenario?
Lohit Shetty Raju, India
L. Shetty Raju, M. Michael, S.T. Binoj, J. Shaji Mathew, R. Menon, G. UnniKrishnan, S. Sudhindran
GI Surgery and Multi Organ Transplant, Amrita Institute of Medical Sciences, India
continuous to be a major cause of post op mortality following Liver transplantation[LT].
Survival in sepsis hinges around early diagnosis & source control.Search for
early marker for sepsis continues .Expression of Fc receptor CD64 on surface of
neutrophils [Ncd64] has been shown in many studies to be an accurate biomarker
in detecting sepsis.
Methods: Prospective observational
study, to evaluate diagnostic accuracy of neutrophil CD64, comparing it
with C-reactive protein (CRP) and procalcitonin (PCT) for the diagnosis of
sepsis in adult patients undergoing LT.Blood samples were collected D -1, D1,
D3, D7. All samples were tested for CRP, PCT & Ncd64 using flow cytometry.Ncd64
values were expressed both as % and mean fluroscence intensity[MFI].Gold
standard for diagnosing sepsis was blood/Drain culture positivity.
Results: Preliminary results from
24 patients were analysed.In 6 patients who were diagnosed sepsis neither
CRP,PCT nor Ncd64 had statistically significant P value in diagnosing
sepsis.But while analysing trend of elevation from Baseline to D7, estimation
of Ncd64 and CRP looks hopeful in predicting sepsis.
Conclusion: As these are preliminary results & study is on-going,
role of Ncd64 is yet to be discovered in
post LT scenario.
|PT03-06 ||Our Strategy to Overcome Hepatic Ischemia Reperfusion Injury -From Bench to Bedside-
Junya Kawasoe, Japan
J. Kawasoe1,2, Y. Uchida1,2, T. Miyauchi1,2, K. Saga1,2, K. Kadono1, H. Hirao1, T. Watanabe3, H. Terajima1,2, S. Uemoto1
1Division of Hepato-Pancreato-Biliary Surgery and Transplantation, Department of Surgery, Graduate School of Medicine, Kyoto University, Japan, 2Department of Gastroenterological Surgery and Oncology, The Tazuke Kofukai Medical Research Institute, Kitano Hospital, Japan, 3Division of Immunology, Institute for Frontier Life and Medical Sciences, Kyoto University, Japan
Background: In the field of liver surgery, especially transplantation, ischemia
reperfusion injury (IRI) causes approximately 10% of early graft failure and leads
to a higher incidence of acute and chronic rejection. It is an urgent issue to overcome IRI from the
viewpoint of organ preservation. This study aims to reveal the molecular mechanism and develop
the effective protective way to
prevent the liver IRI for a safer
Methods :We applied the established partial
warm hepatic IRI model in mice and investigated changes of immune responses due
to IR, and compared the
efficacy of various biological
molecules (neutrophil elastase inhibitor, recombinant Galectin-9, recombinant
thrombomodulin and so on), antioxidant agents (vitamins C and E) and maneuvers
(preoperative short-term fasting). The liver damage was assessed by serum alanine aminotransferase (sALT) level, histological
observations, change in expression profile of various cytokines and analysis of intracellular molecules.
Results: The most effective way to improve IRI was the preoperative short-term (12 hours) fasting. It exhibited a remarkable therapeutic effect
compared with any other drug
or diet we have so far examined.
The up-regulation of Forkhead Box O1 (FOXO-1) induced by the raised acetylated histone and β-hydroxybutyric
acid was a crucial factor for the short-term fasting to ameliorate the liver injury due to IR.
Conclusion: Preoperative short-term dietary restriction might play an
important role to overcome liver IRI and have a therapeutic potential for clinical setting.